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Solubilization Methods The first step intended for the solubilization of a protein is their location in a solution, nevertheless this initial must be introduced from the cellular.For this it is necessary to submit the cell into a lysis method.
Stabilization Once the protein has become removed from it is natural environment, it is actually exposed to many agents that can damage that. the proteins can be affected by p H, temperature, proteases, oxidation of disulphide bridges, contamination by simply heavy mining harvests, salt concentration, etc .
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These tests must be repeated at each step from the purification.
the proteins could be monitored matching to their spectroscopic or fluorescence characteristics, enzymatic assays can be carried out when suitable (protein to become purified sama dengan enzyme).
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